The sensor surface - housed in a plastic carrier to facilitate handling and protect the surface from damage see below - used by BIAcore has two essential features. A coating on the gold layer, providing a means for attachment of the ligand. In the case of the CM5 chip, this is a nm think carboxymethyl-dextran matrix. This matrix provides a a relatively inert hydrophilic environment suitable for most biomolecular interactions. It is easily chemically modified allowing the use of a broad range of well-defined chemistries for covalently coupling of biomolecules to the surface and the negatively charged carboxyl groups allow electrostatic concentration of positively charged molecules from the solution phase. This allows efficient immobilisation from dilute solutions.
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Sensor chip CM3 provides the same functionality as Sensor chip CM5 but has a shorter carboxymethylated dextran matrix. The shorter matrix may be of value in work with large analytes such as cells and virus particles. It may also give reduced non-specific binding in work with serum, and can be valuable in testing the influence of the CM5 dextran matrix on kinetic determinations. Experimental protocols follow the same principles as applied to Sensor chip CM5. Sensor chip CM4 has a dextran matrix like the Sensor chip CM5 but with a low degree of carboxylation.
It provides the same functionality as Sensor chip CM5, but is less negatively charged. The lower degree of carboxylation may be of value for reducing non-specific binding in work with analytes with a high positive charge or crude samples, such as cell culture supernatants and cell homogenates. Sensor chip CM4 may also be useful in kinetic applications where low ligand densities are important. Experimental protocols follow the same principles applied to Sensor chip CM5.
Sensor chip CM5 is a general-purpose chip for interaction analysis involving all types of biomolecules such as small organic molecules, proteins, lipids, carbohydrates, and nucleic acids.
The sensor chip design to allow detailed quantitative studies of these reagents to yield data on e. The ligand is covalently bound to the sensor chip surface via carboxyl moieties on the dextran.
Sensor chip CM5 is regenerated by selective dissociation of the analyte from the covalently immobilized ligand. Conditions should be chosen to achieve complete dissociation of the analyte without affecting the binding characteristics of the ligand.
Sensor chip CM7 is comparable with the CM5 chip but has a higher density of carboxymethylated dextran covalently attached to the gold surface. In addition, the degree of carboxylation is higher which gives the sensor chip an approximately three times higher immobilization capacity. Molecules are covalently coupled to the sensor surface via amine, thiol, aldehyde or carboxyl groups. The surface is ideal for applications, which use small molecules and fragments.
It is generally less suitable when working with large biomolecular analytes. CM3 - short dextran Sensor chip CM3 provides the same functionality as Sensor chip CM5 but has a shorter carboxymethylated dextran matrix.
Sensor chip CM5. CM7 - High carboxylation Sensor chip CM7 is comparable with the CM5 chip but has a higher density of carboxymethylated dextran covalently attached to the gold surface. Details Last Updated: November Contact Forum Search.
Biacore CM chips