CYCLODEXTRIN GLYCOSYLTRANSFERASE PDF

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The enzyme displayed many activities related to the degradation and transformation of starch. Gene encoding H. Sequence analysis revealed an open reading frame of bp that encodes a protein of amino acids. The CGTase is secreted to the extracellular medium by the Tat pathway.

The expression of the CGTase gene yielded a fully active CGTase with similar kinetic behavior to the wild-type enzyme. The H. This site needs JavaScript to work properly. Please enable it to take advantage of the complete set of features! Clipboard, Search History, and several other advanced features are temporarily unavailable.

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Epub Apr PMID: Characterisation of a thermoalkali-stable cyclodextrin glycosyltransferase from the anaerobic thermoalkaliphilic bacterium Anaerobranca gottschalkii. Thiemann V, et al. Arch Microbiol. Epub Aug A novel cyclodextrin glycosyltransferase from Alkaliphilic Amphibacillus sp.

NPST purification and properties. Ibrahim AS, et al. Int J Mol Sci. Engineering of factors determining alpha-amylase and cyclodextrin glycosyltransferase specificity in the cyclodextrin glycosyltransferase from Thermoanaerobacterium thermosulfurigenes EM1. Wind RD, et al. Eur J Biochem. Temperature and pH optima of extremely halophilic archaea: a mini-review. Bowers KJ, Wiegel J. Bowers KJ, et al.

Epub Feb PMID: Review. Show more similar articles See all similar articles. Cited by 7 articles Biotechnological applications of archaeal enzymes from extreme environments. Biol Res. N via mutagenesis of the conserved residues.

Wang H, et al. Epub Jun 8. A novel cyclodextrin glucanotransferase from an alkaliphile Microbacterium terrae KNR 9: purification and properties. Rajput KN, et al. Halophiles: biology, adaptation, and their role in decontamination of hypersaline environments.

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References J Bacteriol. Publication types Research Support, Non-U. Gov't Actions. Cyclization Actions. Electrophoresis, Polyacrylamide Gel Actions. Hydrogen-Ion Concentration Actions. Hydrolysis Actions.

Kinetics Actions. Molecular Weight Actions. Spectrometry, Mass, Electrospray Ionization Actions. Substrate Specificity Actions. Temperature Actions. Substances Starch Actions. Glucosyltransferases Actions. Full-text links [x] Springer. Copy Download.

JRG LANZ VON LIEBENFELS PDF

Cyclodextrin glycosyltransferase

The enzyme displayed many activities related to the degradation and transformation of starch. Gene encoding H. Sequence analysis revealed an open reading frame of bp that encodes a protein of amino acids. The CGTase is secreted to the extracellular medium by the Tat pathway. The expression of the CGTase gene yielded a fully active CGTase with similar kinetic behavior to the wild-type enzyme. The H. This site needs JavaScript to work properly.

LEY 17235 PDF

The YF mutant had the highest affinity which was indicated by the lowest K m of Increasing hydrophobicity around the catalytic center appeared to favor the cyclization activity of the mutants. Cyclodextrin glycosyltransferase EC 2. Catalysis of cyclization is the primary function of CGTase Leemhuis et al. Contrarily, mutant AV CGTase in Bacillus circulans strain improved the hydrolytic activity but reduced cyclization activity Leemhuis et al. Additionally, Dijkhuizen and collages uncovered that mutation of tyrosine , which located in the central active site cleft of CGTase, significantly suppressed the cyclodextrin formation Penninga et al. The amino acid side chain at conserved Phe residues also regulated the hydrolytic activity of CGTase.

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CGTase production, mainly by Bacillus sp. Although, waxy corn starch CS is used industrially to obtain CGTase and CDs because of its high amylopectin content, alternative sources such as amaranth starch AS could be used to accomplish those purposes. The maximum CGTase specific activity Cyclodextrins CDs are synthesized from starch and related carbohydrates such as amylose, amylopectin and maltooligosaccharide by cyclodextrin glycosyltransferase CGTase, E. CGTase catalyzes the CDs formation from starch via inter- and intramolecular transglycosylation reactions, which include cycization, disproportionation, coupling, and hydrolysis.

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