Patient Information Handout. Approximately 85 percent of women of childbearing age in the United States are susceptible to acute infection with the protozoan parasite Toxoplasma gondii. Transmission of T. Some health problems may not become apparent until the second or third decade of life. An estimated to 4, cases of congenital toxoplasmosis occur in the United States each year.

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Toxoplasmosis is caused by infection with the parasite Toxoplasma gondii. It is one of the most common parasitic infections in humans and is most typically asymptomatic.

However, primary infection in a pregnant woman can cause severe and disabling disease in the developing fetus. Recent developments have included increased understanding of the role of parasite genotype in determining infectivity and disease severity.

Risk factors for acquisition of infection have been better defined, and the important role of foodborne transmission has been further delineated. In addition, strategies have emerged to decrease mother-to-child transmission through prompt identification of acutely infected pregnant women followed by appropriate treatment.

Refined diagnostic tools, particularly the addition of immunoglobulin G avidity testing, allow for more accurate timing of maternal infection and hence better decision making during pregnancy. Congenitally infected children can be treated, beginning in utero and continuing through the first year of life, to ameliorate the severity of disease. However, despite these many advances in our understanding of congenital toxoplasmosis prevention and treatment, significant areas of study remain: we need better drugs, well defined strategies for screening of pregnant women, improved food safety, and improved diagnostic tests.

Toxoplasma gondii is a protozoan parasite that infects most species of warm-blooded animals, including humans. Members of the cat family Felidae are the only known definitive hosts for the sexual stages of T gondii and thus are the main reservoirs of infection.

The 3 stages of this obligate intracellular parasite are as follows: 1 tachyzoites trophozoites , which rapidly proliferate and destroy infected cells during acute infection; 2 bradyzoites, which slowly multiply in tissue cysts; and 3 sporozoites in oocysts.

Cats become infected with T gondii by carnivorism or by ingestion of oocysts. Cats that are allowed to roam outside are much more likely to become infected than domestic cats that are confined indoors. After tissue cysts or oocysts are ingested by the cat, sporozoites are released and invade epithelial cells of the small intestine where they undergo an asexual cycle followed by a sexual cycle and then form oocysts, which are then excreted.

Human infection may be acquired in several ways: 1 ingestion of undercooked contaminated meat containing T gondii cysts; 2 ingestion of oocysts from hands, food, soil, or water contaminated with cat feces; 3 organ transplantation or blood transfusion; 4 transplacental transmission; and 5 accidental inoculation of tachyzoites. Transmission through breast milk has not been described. The 2 major routes of transmission of Toxoplasma to humans are oral and congenital.

In humans, ingesting either the tissue cyst or the oocyst results in the rupture of the cyst wall [ 3 ], which releases sporozoites that invade the intestinal epithelium, disseminate throughout the body, and multiply intracellularly. The host cell dies and releases tachyzoites, which invade adjacent cells and continue the process.

The tachyzoites are pressured by the host's immune response to transform into bradyzoites and form tissue cysts, most commonly in skeletal muscle, myocardium, and brain; these cysts may remain throughout the life of the host.

Recrudescence of clinical disease may occur if the host becomes immunosuppressed and the cysts rupture, releasing the parasites. Congenital infection occurs predominantly after primary infection of a pregnant woman. However, well described cases of transmission from women infected shortly before pregnancy, transmission from immunosuppressed women undergoing reactivation, and transmission from women previously infected with one serotype developing a new infection with a second serotype during pregnancy have all been described [ 4 ].

The incidence of congenital toxoplasmosis varies with the trimester during which maternal infection was acquired. For untreated women, the transmission rate is approximately 25 percent in the first trimester, 54 percent in the second trimester, and 65 percent in the third trimester. Although the precise mechanism of movement of the parasite across the human placental is not fully understood, recent studies may offer new insights [ 5 ]. Human placental studies suggest that the extravillous trophoblasts EVT that anchor the placenta to the uterus are much more vulnerable to infection than are the syncytiotrophoblasts that are bathed in maternal blood [ 5 ].

Studies in animals have shown that initial infection occurs in the uterus [ 5 ]. Thus, it is likely that after primary infection a woman becomes parasitemic, leading to intracellular infection in the uterus, which then gradually leads to EVT infection, as the tachyzoites move from cell to cell, with eventual infection of the fetus.

It is also possible that direct movement of tachyzoite infected maternal leukocytes to or across the placenta contributes to fetal infection. It seems likely that multiple mechanisms are involved; which could explain the apparent time delay between primary maternal infection and fetal infection.

In recent studies, researchers have begun to appreciate that differences in 1 reactivation and 2 disease severity may be explained in part by different genotypes of T gondii , of which there are 3 occurring in different parts of the world [ 4 ]. These studies are important because they may explain conflicting reports from different regions of the world on the relative public health importance of screening and treatment for congenital disease.

Further research is needed to define the role of T gondii genotypes and the interplay with human innate immunity, particularly in the developing fetus and newborn infant. One study on transmission across the human placenta showed a trend towards increased transmission by 1 genotype; however, the differences were not statistically significant [ 5 ].

Serologic prevalence data indicate that toxoplasmosis is one of the most common infections of humans throughout the world, and prevalence increases with age [ 6 ]. Due to environmental factors impacting the survival of the oocysts, infection is more common in warm climates and at lower altitudes than in cold climates and mountainous regions. Variations in mode of exposure also lead to variations in prevalence. The incidence of congenital toxoplasmosis depends upon the proportion of women entering pregnancy without prior immunity and the rate of exposure to Toxoplasma during pregnancy.

Estimates of congenital infection in the United States have ranged from 1 in to 1 in 10 live births. Toxoplasmosis can be categorized into 4 groups: 1 acquired in the immunocompetent patient; 2 acquired or reactivated in the immunodeficient patient; 3 ocular; and 4 congenital.

Diagnosis and treatment may be different for each clinical category. In general, diagnosis is accomplished using serologic tests, because isolation of the parasite can be difficult. Molecular testing, such as polymerase chain reaction, plays a critical role in diagnosing infection in the fetus. Acquired infection with Toxoplasma in immunocompetent individuals is generally an asymptomatic infection. The clinical course is benign and self-limited; symptoms usually resolve within weeks to months.

Recent data have suggested an association between T gondii infection and various neurologic or psychiatric syndromes, including schizophrenia, Alzheimer disease, and even suicide [ 8 — 10 ]. These findings are intriguing but require further study to validate. Immunodeficient patients often have central nervous system CNS disease but may have myocarditis or pneumonitis.

In patients with acquired immune deficiency syndrome, toxoplasmic encephalitis is the most common cause of intracerebral mass lesions and is thought to be due to reactivation of chronic infection. Toxoplasmosis in patients being treated with immunosuppressive drugs may be due to either newly acquired or reactivated latent infection [ 11 , 12 ]. Ocular toxoplasmosis, an important cause of chorioretinitis in the United States, may be the result of congenital or acquired infection [ 13 ].

Congenitally infected patients can be asymptomatic until the second or third decade of life, when lesions develop in the eye presumably due to cyst rupture and subsequent release of tachyzoites and bradyzoites. Further defining the interaction of human immunity, timing of infection, and parasite genotype is an important area of ongoing research in understanding ocular toxoplasmosis. The severity of clinical disease in congenitally infected infants is related inversely to the gestational age at the time of primary maternal infection—with first-trimester maternal infection leading to more severe manifestations.

When clinically apparent, it may mimic other diseases of the newborn. In a proportion of cases, spontaneous abortion, prematurity, or stillbirth may result. Involvement of the CNS is a hallmark of congenital Toxoplasma infection.

The presence of chorioretinitis, intracranial calcifications, and hydrocephalus is considered the classic triad of congenital toxoplasmosis. Fever, hydrocephalus or microcephaly, hepatosplenomegaly, jaundice, convulsions, chorioretinitis often bilateral , cerebral calcifications, and abnormal cerebrospinal fluid are the classic features of severe congenital toxoplasmosis.

Other occasional findings included rash maculopapular, petechial, or both , myocarditis, pneumonitis and respiratory distress, hearing defects, an erythroblastosis-like picture, thrombocytopenia, lymphocytosis, monocytosis, and nephrotic syndrome. Current treatment regimens work primarily against the actively dividing tachyzoite form of T gondii and do not eradicate encysted organisms bradyzoites.

Further refining the optimal treatment regimen is an area requiring further research, especially as newer drugs azithromycin, atovoquone, etc with activity against the various stages of Toxoplasma are developed. Continued research is required to determine the impact of therapy during infancy upon recrudescent ocular disease in adolescents who were congenitally infected. Prevention of congenital toxoplasmosis infection involves prevention of primary infection in pregnant women or prevention of transplacental transmission once primary infection has occurred in the mother.

Secondary prevention of severe disease in the infected fetus and newborn relies upon early detection and initiation of appropriate therapy for the child, as described above.

Primary prevention of maternal infection depends upon an understanding of modes of acquisition of infection for a pregnant woman. Risk factors for T gondii infection identified in epidemiologic studies include eating raw or undercooked pork, mutton, lamb, beef, ground meat products, oysters, clams, or mussels and wild game meat, kitten ownership, cleaning the cat litter box, contact with soil gardening and yard work , and eating raw unwashed vegetables or fruits [ 2 , 3 , 14 — 16 ].

These recommendations include the following: 1 whole cuts of meat should be cooked to safe temperatures of at least o F with a 3 minute rest, ground meat should be cooked to o F, and poultry should be cooked to at least o F with a 3 minute rest; 2 fruits and vegetables should be peeled or washed thoroughly before eating; 3 cutting boards, dishes, counters, utensils, and hands should always be washed with hot soapy water after they have contacted raw meat, poultry, seafood, or unwashed fruits or vegetables; 4 pregnant women should wear gloves when gardening and during any contact with soil or sand, because cat waste might be in soil or sand, and wash hands afterwards; 5 pregnant women should avoid changing cat litter if possible.

If no one else is available to change the cat litter, pregnant women should use gloves, then wash their hands thoroughly. Pregnant women should be encouraged to keep their cats inside and not adopt or handle stray cats. Cats should be fed only canned or dried commercial food or well cooked table food, not raw or undercooked meats.

Several outbreaks have been reported in association with drinking untreated water contaminated by oocysts. Freezing meats for several days at subzero 0 o F greatly reduces the risk of infection and may partially explain the declining prevalence of Toxoplasma infection in the United States, because increasing proportions of meats are frozen for prolonged periods before human consumption. An important area of ongoing study is improved food safety—including the role of irradiation, monitored freezing of meats as is done for Trichinella , and development of vaccines for use in cats or intermediate host animals.

Spiramycin available through the US Food and Drug Administration, phone or , or if no response then is recommended for pregnant women with acute toxoplasmosis when fetal infection has not yet occurred, in an attempt to prevent transmission of T gondii from the mother to the fetus [ 17 , 18 ]. Although randomized prospective studies of treatment during acute infection in pregnant women have not been performed, several large prospective and retrospective cohort reviews have been analyzed.

Some researchers have questioned, or been unable to demonstrate, the effectiveness of treatment during pregnancy in preventing congenital infection [ 19 , 20 ] or sequelae in infants [ 20 ]. However, a multicenter observational study found that the treatment of acute T gondii infection in pregnancy was associated with a reduction of sequelae in infants, but not a reduction in maternal-fetal transmission [ 21 ]. Alternative medications, such as azithromycin, have been used but have not been as extensively studied as spiramycin.

Prevention of transmission from a pregnant woman who has experienced primary infection to her fetus requires prompt identification of what is most often an asymptomatic infection in the woman.

Several strategies have been attempted or proposed to identify acutely infected pregnant women. Most strategies involve routine serologic screening for Toxoplasma infection before and during pregnancy.

Two important general areas of ongoing research related to routine screening of pregnant women for Toxoplasma infection have been the refinement of diagnostic tools and the economic impact of strategies to apply those tools. Diagnosing acute infection from a single specimen has proven difficult to accomplish with precision, largely due to the prolonged presence of immunoglobulin Ig M antibodies after acute infection. Most recently, IgG avidity testing has allowed more precise defining of the acuity of infection [ 1 ].

Serial testing once a pregnant woman is found to be IgG negative is accurate but can be costly. Recent models have suggested that it would be cost-effective to screen all pregnant women in the United States [ 22 ] depending upon the cost of the test and the incidence of infection.

From a public health perspective, further refinements of the variation in the epidemiology of toxoplasmosis across populations within the United States are needed to inform decisions regarding universal screening. Congenital toxoplasmosis is a severe disease that can be treated and prevented. Does parasitic genotypic variation explain the variability seen across the globe in disease prevalence and severity? Are there better treatment options available, both for prevention of transmission from an infected pregnant woman to her developing fetus and for the treatment of congenitally infected children, particularly newer drugs that have shown activity against related parasites?

Is it time to seriously consider universal screening of pregnant women in the United States? Do we understand the epidemiology well enough to institute such screening? Can more be done to improve food safety?

In , McCabe and Remington [ 23 ] wrote an editorial stating that the time had come to seriously address toxoplasmosis in pregnancy to prevent congenital infection. Perhaps now the time has really come. Financial support.


Congenital Toxoplasmosis

To estimate the global burden of congenital toxoplasmosis CT , which results from infection of pregnant women with Toxoplasma gondii. The authors systematically searched 9 major databases for published and unpublished sources and established direct contact with the authors of source materials. Searches were country-specific. To be included, studies had to report on the incidence of CT, on positivity to Toxoplasma -specific IgM in infants and pregnant women including seroconversion results or on positivity to Toxoplasma -specific IgG in the general population.


The global burden of congenital toxoplasmosis: a systematic review

Botero D, Restrepo M. En: Botero D, Restrepo M, editores. Parasitosis Humana. Kasper LH. Toxoplasma infection. New York: McGraw-Hill

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